CircRNA_100290 promotes GC cell proliferation and invasion via the miR-29b-3p/ITGA11 axis and is regulated by EIF4A3

Abstract Background Circular RNAs (circRNAs) have been reported to be important regulators of the development and progression various carcinomas. However, role circRNA_100290 in gastric cancer (GC) is still unclear. This study aimed investigate GC invasion metastasis possible underlying mechanism. Methods The expression cells tissues was examined using quantitative real-time polymerase chain reaction (qRT-PCR). cell proliferation, migration, evaluated AGS HGC-27 lines vitro. Bioinformatics tools, dual-luciferase reporter assays, Western blot assays qRT-PCR were used explore pathways downstream circRNA_100290. mechanism regulation explored RNA immunoprecipitation, qRT-PCR, assays. Results significantly upregulated 102 tissues, high closely related Borrmann’s type, lymph node tumour-node-metastasis stage. In vitro, knockdown inhibited invasion. Mechanistically, a assay confirmed direct interaction between miR-29b-3p, which targets ITGA11, an oncogene that epithelial–mesenchymal transition (EMT). addition, EIF4A3, RNA-binding protein (RBP), could inhibit formation by binding flanking sites Low EIF4A3 poor prognosis. Conclusions Elevated promotes EMT via miR-29b-3p/ITGA11 axis might regulated EIF4A3. CircRNA_100290 promising biomarker target for therapy. Graphical abstract